Osteoarthritis (OA) is the most common form of arthritis and the leading cause of chronic disability in the United States (affecting nearly 27 million people). This joint disease impacts the function of multiple tissues, including articular cartilage and subchondral bone, and results in pain, inflammation, and stiffness. Our lab is comparing a chemically-induced small animal model of OA with a surgically-induced model via imaging techniques to quantitatively assess damage in multiple tissues including articular cartilage, subchondral bone, vasculature, and synovium. Additionally, gait and pain analyses are performed to correlate microstructural changes to functional adaptaption. This combination of assays will provide a more complete assessment of the OA environment during disease progression and allow for therapies to be tested in small animal models.